毕节特产珍稀药材圆珠半夏的HPLC指纹图谱分析

为毕节大方圆珠半夏质量标准的建立提供参考,采用HPLC法对10批不同产地及不同采收时间的圆珠半夏进行了指纹图谱研究。结果表明:测定的10批半夏样品有23个共有峰,共有峰面积为91.6%~95.8%,相对峰面积的RSD为0.12%~0.40%;共有峰的相对保留时间从0.182~1.810min,相对保留时间的RSD在0.02%~0.10%。结论,该方法具有良好的稳定性和重复性,可用于圆珠半夏化学成分分析。     

Numerical simulation of deformations and forces of a floating fish cage collar in waves

The dynamic behavior of a fish cage collar in waves was investigated using a numerical model based on the finite element method. The floating collar and mooring system were divided into a series of line segments modeled by straight massless model segments with a node at each end. To verify the validity of the numerical model, research data from other authors were cited and compared with the simulated results, the comparison of results showed a good agreement. The numerical model was then applied to a dynamic simulation of a floating cage collar in waves to analyze its elastic deformation and mooring line tension. The simulated results indicated that the greatest deformation of the collar taken place in the position of the mooring line connection point when incident waves were in the same direction. An increase in the length of mooring line would help to decrease the mooring line tension of the collar. Furthermore, the effects of collar dimension, including collar circumference, pipe diameter in cross-section, and pipe thickness, on the dynamic behavior of the floating collar were discussed. The results of this study provided a better understanding of the dynamic behavior of the fish cage collar.     

Construction of cDNA Library from Populus euphratica

1 Introduction Populus euphratica is a salt tolerant tree species, which is mainly distributed in the desert regions in northwest China (Wei 1993). Moreover, it is the only large tree species, which can survive and develop into forest in these arid and saline-alkali areas. P. euphratica forest plays a very important role in restraining the expansion of desert, maintaining ecological environment in west China, protecting the biological diversity and raising the local people抯 living standard…     

不同工艺参数对竹造纸固剩物/漂珠复合板性能的影响

[目的]研究不同工艺参数（竹造纸固剩物粒度、固剩物与漂珠配比、板材厚度）对复合板材各种性能的影响.[方法]采用正交试验设计,利用竹造纸固剩物和漂珠制造复合板材,并通过对力学性能和导热系数的测定,探讨固剩物粉末粒度、固剩物与漂珠配比、板材厚度对板材性能的影响.[结果]随着粒度和板材厚度的增大,板材的物理力学性能MOE、24hTS呈现下降趋势;随着配比的增大,MOR、MOE增大,24hTS减小.[结论]最佳工艺为固剩物粒度为20～40目、固剩物与漂珠配比为8∶2、板材厚度为14 mm时,此时板材的各种性能达到最优.     

浮动平底推杆等宽凸轮机构第Ⅱ类机构综合问题

以浮动平底推杆等宽凸轮机构为研究对象,通过引入往程、返程、向径标定线和等宽映射区间套等概念,阐述了往程/返程运动角、远近休止角的内在规律性关系,运动学尺寸、行程幅值和初位参数间的内在特征关系,输出摇杆返程角位移规律的求解,主副平底容许选择区段和凸轮基圆半径取值域确定,凸轮基圆半径、压力角和宽度的变化规律等,揭示了等宽凸轮机构的设计蕴含着诸多的异质性内涵。还对等宽凸轮机构、共轭凸轮机构进行了比较,得到了两种形锁合机构相同点、差异点。最后给出等宽凸轮机构的一个综合算例。     

Modelling impact sound reduction in floating wood floors

Abstract

The transmission of footfall-excited impact sound is commonly reduced using floating wood floors. These constructions contain three layers: a load-bearing wood floor, a resilient material and a floating wood slab. For wood construction excited by transient load (e.g. footsteps), sound reduction estimations may contain uncertainties due to inadequate modelling of footsteps and floor components. This paper presents a multiple degree-of-freedom system model using Working Model 2D® (WM2D). The model includes orthotropic behavior of wood and equivalent spring stiffness determination of the resilient layer. Sound reduction is determined and it is shown that larger reductions are possible through the inclusion of floating wood slabs in the load-bearing floor.     

Antigen-nonspecific macrophage factors modulating the antibody response in vitro

Antibody response to an antigen involves the co-operation between three types of cells: macrophages, T cells and B cells. The cognate interactions between these cells play a fundamental role in the expression of a specific antibody response, but the last is modulated by antigen-nonspecific soluble factors produced either by macrophages or by T cells. Macrophages elaborate a spectrum of molecules modulating the function of lymphoid cells; among them are IL1 and prostaglandins of the E series, which are respectively enhancer and inhibitor of the antibody response in vitro. These molecules alter T cell and B cell activities through different mechanisms involving activation or inhibition of IL2 production, or alteration of cells surface antigens. However, the cellular events following the fixation of soluble factor on its receptors are not known.     

刺参微卫星DNA分子标记的分离及筛选

采用磁珠富集法分离刺参Apostichopus japonicus的微卫星分子标记,共获得阳性克隆123个,其中106个含微卫星DNA序列。分析结果表明:完美型有48个,占45.28%,非完美型有39个,占36.79%,复合型有19个,占17.92%;重复碱基数以双核苷酸重复最常见,占84.91%,双核苷酸中又以CA/GT重复所占比例最大;在重复次数方面,以重复数为3~10次、11~18次和≥35次最为常见,各占39.62%、26.42%和16.98%,重复数达100次以上的有5个,最多的达到148次,有28个微卫星序列微卫星含量丰富。筛选的22对引物中,可产生出扩增产物的有17对,其中16对引物的产物带在预测区域内出现;有7对引物在中国、俄罗斯刺参模板中表现出多态性。文中还对刺参微卫星的特点进行分析,对刺参微卫星引物的PCR筛选结果进行了探讨。     

用磁珠富集法制备史氏鲟的微卫星分子标记

采用生物素-磁珠富集法与放射性同位素杂交法相结合的方式,研究了史氏鲟Acipenser schrencki基因组的微卫星资源。结果表明:在得到的1 400多个细菌中,有300多个阳性克隆,将其中96个进行测序,在这些序列中共有115个微卫星核心序列,含99个重复次数大于10次的微卫星核心序列。其中完美型标记40个,占40.4%;非完美型标记52个,占52.5%;混合型标记共7个,占7.1%。随机选取侧翼序列较长的50个序列,依据引物设计原理设计引物50对,经3次PCR筛选,有28对引物可得到稳定的特异性扩增;同时利用史氏鲟的6个个体检验了这些位点的多态性,并统计了等位基因数,发现有22对等位基因具有良好的多态性。     

烟草漂浮育苗培养基质及营养液对烟苗生长发育的影响

为优化烟草漂浮育苗培养基质和营养液配方，进行了共计7个处理的试验。结果表明：以一定比例的碳化谷糠、泥炭、蛭石和膨化珍珠岩配成的基质有利于培育壮苗。营养液则以一定比例的硝酸铵、硫酸铵、尿素和微量元素的配方较好。